久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 恒遠產(chǎn)品文獻:試劑霍亂毒素引用文獻

恒遠產(chǎn)品文獻:試劑霍亂毒素引用文獻

更新時間:2020-12-29   點擊次數(shù):954次

【文獻標題】Sphk1 participates in malignant progression of breast cancer by regulating epithelial-mesenchymal transition and stem cell characteristics

【作者】Zhijie Chen, Bingxiong Liu

【作者單位】漢川市人民醫(yī)院(Hanchuan People’s Hospital)

【文獻中引用產(chǎn)品】

霍亂毒素 CAS號:9012-63-9

【關(guān)鍵詞】Breast cancer,Sphingosine kinase 1,Epithelial-mesenchymal transition,Stem cell,Metastasis

【DOI】doi.org/10.1016/j.tice.2020.101380

【影響因子(IF)】2.90

出版期刊】《Tissue and Cell》

【產(chǎn)品原文引用】

Cell and culture

Human breast epithelial cells (MCF-10A, CRL-10317) and BC cell lines MCF-7 (CRL-3435), T47D (HTB-133), SKBR3 (HTB-30), MDA-MB-231 (HTB-26), BT-474 (HTB-20) were purchased from American Tissue Culture Collection. The cells, except MDA-MB-23 cells, were cultured in DMEM (12491015, ThermoFisher, USA) containing 10 % FBS (30067334, ThermoFisher, USA), 1% penicillin/streptomycin (15140163,ThermoFisher, USA) at 37 °C in 5% CO2. MCF-10A cell line was cultured with additional 100 ng/mL cholera toxin (Hengyuan,China,hyswbio) on the basis of other cell culture condition. After culture for one day, the original medium was discarded, and the cells were washed 1–2 times by PBS. Next, 5 mL fresh DMEM medium was added and further incubated with the cells in a CO2 incubator. When the confluence reached 80%–90%, the cells were subcultured. MDA-MB-231 cells were cultured in Leibovitz’s L15 medium (11415064, ThermoFisher, USA) without CO2。

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務員!

更多公司福利請關(guān)注“恒遠生物”VX公眾號!

 

聯(lián)


特级无码毛片免费视频尤物| 无码av无码天堂资源网| 永久免费av无码入口国语片| 最近免费中文字幕| 风韵丰满熟妇啪啪区老熟熟女| 老色鬼久久亚洲av综合| 日本丰满熟妇hd| 末发育娇小性色xxxxx视频| 国产性猛交╳xxx乱大交| 亚洲欧洲中文日韩久久av乱码| 躁躁躁日日躁| 18禁黄无遮挡网站| 久久久久久人妻一区精品| 妺妺窝人体色www聚色窝| 久9re热视频这里只有精品| 精品无码一区二区三区爱欲| 西西午夜无码大胆啪啪国模| 国产精品无码dvd在线观看| 精品国产av 无码一区二区三区| 精品人妻伦一二三区久久| 人妻无码中文专区久久五月婷| 中文亚洲av片不卡在线观看| 免费人成网站视频在线观看国内| 亚洲自偷自偷偷色无码中文| 婷婷五月深深久久精品| 国产色在线 | 日韩| 国产男小鲜肉同志免费| 精品无人区一区二区三区| 97免费人妻无码视频| 欧美牲交a欧美牲交aⅴ免费真| 精品女同一区二区| 色哟哟最新在线观看入口| 精品国产一区二区三区久久| 妓院一钑片免看黄大片| 2019年最好看的中文免费视频| 精品少妇人妻av免费久久洗澡| 熟女体下毛毛黑森林| 曰批免费视频播放免费直播| 亚洲成av人片乱码色午夜| 国产女主播喷水视频在线观看| 欧美激情精品久久|