久久久久久av无码免费网站下载,一本加勒比hezyo无码资源网,久久久久黑人强伦姧人妻,好爽毛片一区二区三区四

技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 雞分泌型免疫球蛋白A,雞白細胞介素2 ELISA試劑盒引用文獻

雞分泌型免疫球蛋白A,雞白細胞介素2 ELISA試劑盒引用文獻

更新時間:2021-01-07   點擊次數(shù):1016次

【文獻標題】Effects of Taishan Pinus massoniana pollen polysaccharide on the subunit vaccine of Proteus mirabilis in birds

【作者】Guolin Cui,Shixun Zhong,Shifa Yang,et.al

【作者單位】山東農(nóng)業(yè)大學(Shandong Agricultural University)

【文獻中引用產(chǎn)品】

雞分泌型免疫球蛋白A(sIgA)ELISA試劑盒

雞白細胞介素2(IL-2)ELISA試劑盒

【關(guān)鍵詞】Taishan Pinus massoniana pollen,polysaccharide,Subunit vaccine,Proteus mirabilis,Adjuvant,Effect

【DOI】dx.doi.org/10.1016/j.ijbiomac.2013.02.006

【影響因子(IF)】4.97

出版期刊】《International Journal of Biological Macromolecules》

【產(chǎn)品原文引用】

2.4. Immunization

Threehundred1-day-oldchicks were randomlydividedintofive groups (n = 60 per group). Groups I–V were injected subcutaneously with 0.2 ml of WO-OMP vaccine, PP-OMP vaccine, TPPPS-OMP vaccine, OMP-only vaccine and physiological saline, respectively, at 3,7 and 12 days old. The final concentrations of OMP were 500 g/ml at the first vaccination, 1 mg/ml at the second vaccination and 2 mg/ml at the third vaccination. Seven days after the third vaccination, 30 chicks from each group were challenged with 100 LD50 P. mirabilis strain Q1 by oral administration.

On days 3, 7, 14, 21, 28, 35, 42 and 49 after the first vaccination,the peripheral blood and duodenum were sampled for analysis.The antibody titers were detected using indirect enzyme-linked immunosorbent assay (ELISA), T-lymphocyte proliferation rates were detected with flow cytometry and secreting-type IgA (SIgA)and interleukin-2 (IL-2) were measured using ELISA kits (Shanghai Hengyuan Bio-technology Co. Ltd.). The pathogenesis and survival status of chicks were clinically examined daily for 14 successive days after challenge. The mortality, morbidity and protective rates in each group were calculated as follows:


完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠生物”VX公眾號!

聯(lián)


国产精品成人99一区无码| 无码人妻精品一区二| 99久久精品费精品国产一区二区| 肉大捧一进一出免费视频| 国产av丝袜旗袍无码网站| 国产suv精品一区二区883| 末成年女av片一区二区| 夜夜嗨av一区二区三区| 成人免费无码大片a毛片软件| 久久青青草原一区二区| 欧美极品jizzhd欧美| 每日更新在线观看av| 99精品国产一区二区三区不卡| 无码av波多野结衣久久| 996久久国产精品线观看| 无码无套少妇毛多18pxxxx| 国产精品人人做人人爽人人添| 狠狠色噜噜狠狠狠777米奇小说 | 久久精品国产99久久久| 中国老熟妇自拍hd发布| 国产成人免费a在线视频| 无套内谢老熟女| 亚洲精品一品区二品区三品区 | 国产主播一区二区三区在线观看| 九月婷婷人人澡人人添人人爽| 国内少妇人妻丰满av| 狼色精品人妻在线视频| 国精产品一区一区三区有限在线 | 国农村精品国产自线拍| 精品无码中文视频在线观看| 国产女主播精品大秀系列| 两口子交换真实刺激高潮| 精品人妻av一区二区三区| 中文字幕无码乱码人妻系列蜜桃| 被三个男人绑着躁我好爽视频 | 99re热这里只有精品视频| 亚洲av永久无码精品| 国产精品白浆一区二小说| 精品国产污污免费网站入口| 好日子在线观看视频大全免费动漫| 久久人妻少妇嫩草av蜜桃|