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人β細(xì)胞素(BTC)

更新時(shí)間:2022-08-11

簡要描述:

上海恒過生物長期代理美國進(jìn)口分裝、原裝ELISA“人β細(xì)胞素(BTC) ",現(xiàn)貨供應(yīng),*,質(zhì)量保證。中英文雙版說明書,公司提供免費(fèi)代測服務(wù)。歡迎您來電來涵.

型號(hào):HY21323E點(diǎn)擊量:680

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試劑名稱:人β細(xì)胞素(BTC)

英文名稱:Human beta cellulin,BTC ELISA KIT

檢測范圍:詳見說明書

產(chǎn)品規(guī)格:96人份/48人份

檢測目標(biāo):人、大小鼠、豬、豚鼠、雞、羊、兔...

保存條件: 2-8℃

應(yīng)用范圍:科研用檢測試劑
人β細(xì)胞素(BTC) Assay procedure(操作步驟)
1.Dilute and add sample:Dilute Original density Standard as follow table:
8 pg/ml 5 Standard 150μl Original density Standard+150μl Standard diluent
4pg/ml 4 Standard 150μl 5 Standard+150μl Standard diluent
2 pg/ml 3 Standard 150μl 4 Standard+150μl Standard diluent
1 pg/ml 2 Standard 150μl 3 Standard +150μl Standard diluent
0.5 pg/ml 1 Standard 150μl 2 Standard +150μl Standard diluent
2.add sample:Set blank wells separay (blank comparison wells don’t add sample and HRP-
Conjugate reagent, other each step operation is same). testing sample well. add Sample
dilution 40μl to testing sample well, then add testing sample 10μl (sample final
dilution is 5-fold), add sample to wells , don’t touch the well wall as far as
possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold (or 20-fold) with distilled
water and reserve.
5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate:Operation with 3.
8.washing:Operation with 5.
9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
light preservation for 15 min at 37℃
10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color
change to yellow color).
11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution
and within 15min.
人β細(xì)胞素(BTC) 相關(guān)產(chǎn)品:
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上海恒遠(yuǎn)生物長期提供酶免Elisa試劑盒、ELISA檢測試劑盒、免疫組化試劑盒、人Elisa試劑盒、大小鼠Elisa試劑盒、抗體抗原,價(jià)格實(shí)惠,質(zhì)量有保證,信譽(yù)*。凡購買目錄本公司ELISA檢測試劑盒可免費(fèi)提供代測服務(wù)。更多Elisa試劑盒,咨詢:,.

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